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International Board
Manuel Espinosa, Spain
Laura Frost, Canada
Donald Helinski, USA
Clarence Kado, USA
Richard Novick, USA
David Romero, Mexico
Christopher M. Thomas, UK
Suggested Time Schedule
(Download the Time Schedule in MS Word or PDF
format here.)
Tuesday 19. 9.
| Arrival of participants, registration, accommodation and welcome reception in TOP HOTEL PRAHA, Blazimska 1781/4, CZ-149 00 Praha 4. |
Wednesday 20. 9.
| 7.30 - 8.30 | Breakfast in hotel |
| 8.30 | Transport to the Congress Center IKEM by 3 buses (cca 15 min) |
| 9.00 - 9.30 | Opening talks |
| REPLICATION (chairs: M. Espinosa, C. M. Thomas) | |
| 9.30 - 10.30 | Biochemistry and Cytology of Plasmid Behavior. Donald R. Helinski, Ron Caspi, Joe Pogliano, Marcin Pacek, Zhenping Zhong, Thanh Ho, Giac Consiglieri, Yong Jiang, Aresa Toukdarian, and Igor Konieczny |
| 10.30 - 11.00 | Chaperone Activities during Plasmid RK2 DNA Replication. Igor Konieczny, Marcin Pacek, Anna Saari and Krzysztof Liberek |
| 11.00 - 11.30 | The Plasmid RK2 Replicon: Submembrane Localization in E. coli, Interaction of the Initiator Protein TrfA with a New Host Component, and Distribution in the Membrane of other Gram Negative Species. W. Firshein, P.D. Kim and T. Banack |
| 11.30 – 12.15 | Coffee and installation of posters |
| 12.15 – 12.45 | On the Structure of the CopA-CopT Complex Involved in Regulation of Plasmid R1 Replication, its Pathway and Kinetics of Formation. E.G.H. Wagner, F. Kolb, H.M. Engdahl, C. Slagter-Jäger and P. Romb |
| 12.45 – 13.15 | Structural and Functional Similarities between the Initiator Proteins from Bacterial Plasmids (RepA) and Eukaryotes (Orc4p) Rafael Giraldo |
| 13.15 – 13.45 | Yeast Forward and Reverse Two Hybrid Approachs to Analyze Protein-Protein Interactions during Initiation and Termination of Replication. Deepak Bastia, Aardra Potnis, Rahul Sharma and Sashidhar Mulugu |
| 13.45 – 15.00 | Lunch (Congress Center) |
| 15.00 – 15.30 | Control of DNA Strand Opening at the g Origin of the Plasmid R6K. R. Krüger and M. Filutowicz |
| 15.30 – 15.50 | Initiation of DNA Replication and its Regulation in Plasmid ColE2. T. Itoh, M. Yagura, S. Nishio, M. Han, T. Nagase and K. Ogiwara |
| 15.50 – 16.20 | Plasmid pIP501 Encoded Transcriptional Repressor CopR: the Structured Acidic C-terminus is not Required for DNA-Binding or Dimerization, but for Protein Stability. Kornelia Kuhn, Katrin Steinmetzer and Sabine Brantl |
| 16.20 – 16.50 | Characterisation of the Streptococcus pyogenes Plasmid pSM19035-encoded w Transcriptional Repressor. Ana B. de la Hoz, Rolf Misselwitz, Silvia Ayora, Karin Welfle, Silvia Fernández, Christian Speck, Heinz Welfle, Wolfram Saenger and Juan C. Alonso |
| 16.50 – 17.10 | Coffee |
| 17.10 – 18.10 | The pT181 Replicon after 21 Years of Study: Rolling Circle Plasmid Replication Comes of Age. Christopher D. Thomas, Fotios Papadopoulos & Kelly Smith |
| 18.10 – 18.40 | Initiator Protein-Origin Interactions during the Initiation and Termination of Plasmid pT181 Rolling-Circle Replication. Tseh-Ling Chang, M. Gabriela Kramer and Saleem A. Khan |
| 18.40 – 19.00 | Features of the CopG Regulatory Protein Encoded by Plasmid pMV158 Ana M. Hernández-Arriaga, F.Xavier Gomis-Rüth, Manuel Espinosa, Miquel Coll and Gloria del Solar |
| 19.00 – 19.20 | Discoveries within the Seven Plasmid Array of Bacillus megaterium QM B1551. M. Kunnimalaiyaan, Y. Zhou, M. Scholle, G. Baisa and P.S. Vary |
| 19.20 – 20.15 | Dinner in Congress Center |
| 20.15 – 22.00 | Poster Session |
| 22.00 | Transport to hotel by 3 buses
|
| 7.30 - 8.30 | Breakfast in hotel |
| 8.30 | Transport to the Congress Center IKEM by 3 buses (cca 15 min) |
| REPLICATION (chairs: M. Espinosa, C. M. Thomas) | |
| 9.00 - 9.30 | Replication-Induced Transcription of an Autorepressed Gene: the Replication Initiator Gene of Plasmid P1. Suman Mukhopadhyay and Dhruba K. Chattoraj |
| 9.30 – 9.50 | The Protelomerase of Temperate Escherichia coli Phage N15 Has Cleaving-Joining Activity. Erich Lanka, Guenter Ziegelin, Jan Deneke and Rudi Lurz |
| 9.50 – 10.10 | Structure-Function Relationship of Plasmid RSF1010 Helicase RepA. Günter Ziegelin, Timo Niedenzu, Wolfram Saenger, Rudi Lurz, and Erich Lanka |
| 10.10 – 10.30 | Single-copy/High-copy (SC/HC) pBAC/oriV Novel Vectors for Genomics and Gene Expression. Jadwiga Wild, Zdenka Hradecna and Waclaw Szybalski |
| 10.30 – 10.50 | Coffee |
| HORIZONTAL GENE POOL (Chairs: K. Smalla, H. Richards) | |
| 10.50 – 11.50 | Plasmid Mediated Multidrug Resistance. Ronald A. Skurray and Melissa H. Brown |
| 11.50 – 12.20 | Does Dental Amalgam Select for Mercury and Antibiotic Resistant Bacteria? Hilary Richards |
| 12.20 – 12.50 | PCR-based Detection of BHR-plasmids and Gentamicin Resistance Genes in Community DNA. Kornelia Smalla, Renata Krasowiak, Holger Heuer, Ellen Krögerrecklenfort, and Christopher Thomas |
| 12.50 – 13.10 | Combining Plasmids and Bacterial Diversity Studies in Soil Microbial Communities. K. Drønen, E. de Faveri, G. Rist, E. Top, V. Torsvik and L. Øvreås |
| 13.45 – 15.00 | Lunch in Congres center |
| 15.00 – 18.30 | Facultative guided tour of Prague |
| 20.00 – 21.30 | Concert of classical music in the baroque church of St. Nicholas
|
| 7.30 - 8.30 | Breakfast in hotel |
| 8.30 | Transport to the Congress Center IKEM by 3 buses (cca 15 min) |
| TRANSFER (Chairs: R. Skurray, S. Farrand) | |
| 9.00 – 10.00 | The Role of VirB Proteins in T-pilin and T-pilus Biogenesis in Relation to Flagellation of Agrobacterium tumefaciens. Erh-Min Lai , Olga Chesnokova, Lois M. Banta, and Clarence I. Kado |
| 10.00 – 10.30 | Maturation of IncP Prepilin. Erich Lanka, Ralf Eisenbrandt, Markus Kalkum and Rudi Lurz |
| 10.30 – 11.00 | Binding of the Essential DNA Transfer Protein TraM to the oriT of the Conjugative Resistance Plasmid R1. Günther Koraimann |
| 11.00 – 11.20 | Coffee |
| 11.20 – 11.50 | The IncF Relaxosome Protein TraM Performs a Transfer Function that Is Essential at a Stage Following oriT Cleavage. Martina Bamberger, Heimo Strohmaier, Günther Koraimann and Ellen L. Zechner |
| 11.50 – 12.20 | Recognition of OriT by MobA, the Strand Cleaving-Rejoining Protein for Conjugal Transfer of R1162. Eric Becker, Shuyu Zhang and Richard Meyer |
| 12.20 – 12.50 | DNA Recognition of F Factor TraI and TraY: Trai Is the Major Determinant of Relaxosome Specificity. Joel F. Schildbach, Jennifer C. Stern, Pamela L. Lum, Lara M. Street and Matthew J. Harley |
| 12.50 – 13.20 | Architecture of the R388 DNA Mobilization Machine. Gabriel Moncalián, Mikel Hernando, María Lucas, Itsaso Hormaeche, Itziar Alcorta, Félix Gońi, Xabier Gomis, Miquel Coll and Fernando de la Cruz |
| 13.45 – 15.00 | Lunch in Congress center |
| 15.00 – 15.30 | Conjugal Transfer of Plasmid pMV158: Features of the Plasmid –Encoded MobM Protein and its oriT Target DNA. María Eugenia Farías and Manuel Espinosa |
| 15.30 – 16.00 | Plasmid Conjugation in Bacilli and Kinetics of Transfer. Gert B. Jensen, Lars Andrup, Lasse Smidt, Andrea Wilcks and Yuan Zhiming |
| 16.00 – 16.30 | pSAM2, an Integrative Mobile Genetic Element from Streptomyces. J.-L. Pernodet, C. Possoz, A. Raynal, A. Friedmann, J. Gagnat and M. Guérineau |
| 16.30 – 16.50 | Transfer of the Integrative Element, pSAM2, of Streptomyces ambofaciens. Christophe Possoz, Josette Gagnat, Michel Guérineau and Jean-Luc Pernodet. |
| 16.50 – 17.10 | Coffee, then free time (possible free discussion at posters)
|
| 7.30 – 8.30 | Breakfast in hotel |
| 8.30 | Transport to the Congress Center IKEM by 3 buses |
| MAINTENANCE AND STABILITY (Chairs: R. Diaz, J. Mahillon) | |
| 9.00 – 10.00 | Partitioning and Maintenance of Bacterial Plasmids. Christopher M Thomas, Colin Bignell, Lewis Bingle, Anthony Haines, Kalliope Kostelidou, Richard Lewis, Wei Zeng and Malgorzata Zatyka |
| 10.00 – 10.30 | Domain Mapping of KorB of RK2 Plasmid: KorB-IncC Interaction Region. M. Lukaszewicz, K. Kostelidou, C.M. Thomas and G. Jagura-Burdzy |
| 10.30 - 10.50 | A Protein-Protein Interaction Domain Shared by the KorA, TrbA, and KlcB Proteins of Promiscuous Plasmids of Incompatibility Group P. Anuradha Bhattacharyya and David H. Figurski |
| 10.50 – 11.10 | Coffee |
| 11.10 – 11.40 | The Parf Subgroup of the ParA Superfamily of ATPases: a Widespread Mechanism by which Genome Segregational Stability Is Achieved? Finbarr Hayes |
| 11.40 – 12.10 | Segregation Dynamics of the E. coli Chromosome and the P1 Plasmid. Therese Brendler, James Sawitzke, Yongfang Li and Stuart Austin |
| 12.10 – 12.40 | P1 Plasmid Partitioning: Pairing by Centromere-Binding Protein ParB. Michael Yarmolinsky, Oleg Rodionov and Rotem Edgar |
| 12.40 – 13.45 | Poster session |
| 13.45 – 15.00 | Lunch in Congress center |
| Programmed Cell Death Takes a Turn. Jakob Møller-Jensen, Thomas Franch, Kim Pedersen, Marie Mikkelsen, Susanne Christensen, and Kenn Gerdes | |
| 16.00 – 16.30 | Regions Involved in Functional Interactions Between the Bacterial Toxin Kid and the DnaB Helicase. Ramón Díaz-Orejas |
| 16.30 – 16.50 | Coffee |
| 16.50 – 17.20 | Autoregulation of the ccd Poison/Antidote System Hassan Afif, Martine Couturier and Laurence van Melderen |
| 17.20 – 17.50 | The post-Segregational Killing System of the Streptococcal Plasmid pSM19035. Urszula Zielenkiewicz, Izabela Sitkiewicz, Izabella Kern-Zdanowicz and Piotr Ceglowski |
| 17.50 – 18.20 | Plasmid Stabilization Via post-Segregational Killing: a Gram Positive Perspective. Keith E. Weaver, Tony Greenfield, Erik Ehli, Todd Kirschenmann, Mark Heine, and Kristi Walz |
| 19.00 – 20.00 | Dinner in Congress center |
| 20.00 – 22.00 | Poster Session |
| 22.00 | Transport to the hotel by 3 buses
|
| 7.30 – 8.30 | Breakfast in hotel |
| 8.30 | Transport to the Congress Center IKEM by 3 buses |
| EVOLUTION AND DIVERSITY (Chairs: C. Kado, W. Szybalski) | |
| 9.00 – 10.00 | Evolution of the 'Mobilome': Commonality and Diversity of Structure and Function within Mobile Genomes. A. Mark Osborn |
| 10.00 – 10.30 | Prokaryotic Insertion Sequences: New Trends in the Collection "2000". Jacques Mahillon, Catherine Léonard, Céline Vermeiren and Mick Chandler |
| 10.30 – 11.00 | DNA Restriction-Modification: Selfish Mobile Elements that Have Maintained and Rearranged Bacterial Genomes. Ichizo Kobayashi |
| 11.00 – 11.20 | Coffee |
| 11.20 – 11.50 | Comparison and Evolution of IncQ and IncQ-Like Plasmids. Douglas E. Rawlings and Murray N. Gardner |
| 11.50 – 12.10 | Molecular Evolution/Adaptation in the Initiation Apparatus of Drug Resistance Plasmid R6K DNA Replication. Manabu Inuzuka, Yukie Matsui, Yuko Nakamura and Seiichi Yasuda |
| 12.10 – 12.40 | Development of Environment-Specific Replicon Probes to Determine Prevalence and Diversity of Marine Plasmids. Patricia Sobecky, Marisa Cook and Deana Erdner |
| 12.40 – 13.45 | Poster Session |
| 13.45 – 15.00 | Lunch in the Congress center |
| 15.00 – 16.00 | Complete Nucleotide Sequence and Analysis of pTiBo542: What Genomics Tells us about Structure and Evolution of Plasmids in the Family Rhizobiaceae. Philippe Oger, Claudia Reich, Gary J. Olsen, and Stephen K. Farrand |
| 16.00 –16.30 | At the Crossroads between Replication and Recombination: Region-Specific Hyperrecombination in the Symbiotic Plasmids of Rhizobium. Edgar Valencia-Morales, Araceli Dávalos and David Romero |
| 16.30 – 16.50 | Inter-Plasmid Recombination Leads to the Generation of New Symbiotic Plasmids. Susana Brom, Lourdes Girard, Cristina Tun-Garrido and David Romero |
| 16.50 – 17.10 | Coffee |
| 17.10 – 17.30 | Isolation and Molecular Analysis of the Conjugative Broad-Host-Range Mercury Resistance Plasmid pS2. Susanne Schneiker, Alfred Pühler and Werner Selbitschka |
| 17.30 – 17.50 | Molecular Diversity of Plasmids Bearing Genes for Toluene and Xylene Metabolism in Pseudomonas Strains Isolated from Different Contaminated Sites in Belarus. Vladimir S. Sentchilo, Alexander J. B. Zehnder and Jan Roelof van der Meer |
| 17.50 – 19.00 | Conclusion of Symposium |
| 19.15 | Transport to the center of Prague by 3 buses |
| 20.00 – 23.00 | Farewell dinner in brewery "U Fleku" |
| 23.00 | Transport by 3 buses to the hotel
|
| Departure of participants |